15 December 2021, Volume 21 Issue 4

    

• Crop Science
• CRISPR/Cas9 editing and expression analysis of LOC_Os10g05490 in rice under cold stress

  SHEN Chunxiu

  2021, 21(4): 1-10. https://doi.org/10.3969/j.issn.1004-1524.2017.01.01

  Abstract ( ) PDF ( )

  To explore the expression and cold tolerance function of LOC_Os10g05490 in rice under cold stress, semi-quantitative analysis was performed for Dongxiang wild rice and 93-11. The results showed that expression level of LOC_Os10g05490 in 93-11 was the same before and after cold stress, but it was down regulated in Dongxiang wild rice after cold stress. This was consistent with RNA-seq data and further study indicated that this gene might be associated with cold tolerance in Dongxiang wild rice. Plant expression vectors with CRISPR/Cas9 genome editing technique were subsequently constructed to target the LOC_Os10g05490 locus, and were transformed into the japonica rice variety TP309. A total of 26 transgenic positive plants were obtained. This study may lay the foundation for subsequent screening of mutant plants and identification of genes associated with cold tolerance in rice.