LUO Junyi, YING Congping, WANG Yinping, LIU Silei, LIU Kai
With the continuous progress of ecological civilization construction, fishery resource conservation in Huaihe River Basin has been paid more and more attentions. Fish health is closely related to fish resources with the intestinal flora as a key part. However, the report on the characteristics of fish intestinal flora in Huaihe River Basin is very scarce. To explore the composition and influencing factors of fish intestinal flora in Anhui section of Huaihe River, in March 2019, 90 intestinal samples were collected from 6 fish species, Acheilognathus chankaensis, Abbottina rivularis, Coilia nasus, Hemiculter bleekeri, Pelteobaggrus nitidus,and Parabramis pekinensis), and analyzed using 16S rRNA high-throughput sequencing technologies. Result display, Proteobacteria, Cyanobacteria and Verrucomicrobiota were the dominant bacteria in the gut of the six fish species. Furthermore, Bacteroidetes and Firmicutes also occupy a relatively high proportion in fish gut. Affected by feeding habits, the flora structure each group had significant difference (P<0.01). LEfSe analysis found that the intestinal flora of herbivorous fish species were enriched into 46 iconic differentiating bacteria including Aminobacter, Planctomicrobium, Blastopirellula among others. Pseudomonas and Sphingomonas had significant differences in the intestinal tracts of fish in different resident water layers, and a large number of distinctive differential bacteria were enriched in the fish species of bottom water layer where 17 species of bacteria such as Arenimonas, Blastopirellula and SH-PL14 were documented. In addition, there was no significant difference in the dominant bacteria genera of the fish intestinal tracts among different reaches, but many rare groups were found in the fish intestinal tracts of the middle reaches. In general, the composition of fish intestinal flora is closely related to feeding habits, habitat water layer and habitat reaches, among which phytopherbivorous fish, benthic fish and midstream fish showed obvious differences between their respective groups. Our studies clarified potential influencing factors of fish intestinal flora in the natural environment, provided data support for the establishment of fish intestinal flora database and new ideas for the conservation of fish resources in Huaihe River.