Studies on anesthetic effect of eugenol on Oplegnathus fasciatus juvenile
CHEN Lu, WANG Haoxue, ZHAO Jianping, LI Zhe, XU Kaida, LYU Zeyan
In order to explore the anesthetic effect of eugenol on juvenile Oplegnathus fasciatus, the effects of eugenol at different anesthetic concentrations on this fish species were studied, and described using anesthesia time, recovery time, survival rate and air exposure time, aiming to provide a reference for marker release, live transportation, production and breeding of O. fasciatus. When the concentration of eugenol was less than 20 mg/L, the ideal anesthetic effect cannot be achieved. When the concentration of eugenol was more than 30 mg/L, the anesthetic time decreased with the increase of concentration. With the prolongation of recovery time, all of them can be completely resuscitated within this range. The time required to enter deep anesthesia and recovery varied between 75.67 and 149 s and between 399.67 and 495 s, respectively, and the survival rate reached 100%. Among them, death occurred at 90 mg/L, and the suitable anesthetic concentration ranged from 30 to 80 mg/L. Within the range of effective anesthesia concentrations, the survival rate reached more than 93% within 10 min of anesthesia. With the increase of time, the higher the anesthesia concentration, the lower the survival rate of fish. The survival rate decreased to less than 50% at 20 min and 30% at 30 min, and the survival rate decreased significantly. Under the condition of 30 mg/L, the juvenile of O. fasciatus can be fully recovered within 12 min after entering ANES 5, and the recovery time reached 765.33 s at 12 min, not in line with the ideal recovery time. The suitable operation time should be less than 10 min. Studies have shown that in practical applications, attention should be paid to the time of soaking and air exposure. If the operation time is too long, the concentration of eugenol should be appropriately reduced to ensure the survival rate of fry.