Characterization of microbial communities in constructed wetlands and prawn culture systems
JIN Tongtong, ZHU Shengmin, CUI Zhengguo, SHAN Baotian, XU Jia
In order to explore the performance of constructed wetlands in treating seawater shrimp tail water, and the distribution pattern of microbial communities among and between plant roots and different substrates, in this study, we constructed a composite vertical subsurface flow constructed wetland to study the removal effect of pollutants in seawater shrimp tail water, and characterized the microbial community structure of constructed wetlands using high-throughput sequencing technology. The results showed that the system has a high removal effect on nutrients. The removal rate of COD, NH+4-N, NO-2-N and NO-3-N of the shrimp tail water by the CW selected in this study reached 41.370 ± 6.370%, 70.330 ± 6.040%, 73.810 ± 7.140% and 67.260 ± 7.570%, respectively, and the removal effect of the downstream pool was better than upstream pool. The composition of microbial communities in plant roots and substrates was similar, but there were significant differences in diversity. Compared with plants and other substrates, the diversity of microbial communities in coral layer was the lowest. Proteobacteria, bacteroidota, Firmicutes, actinobacterota and desulfurobacteria were the dominant phylum. α-Alphaproteobacteria, γ-Gammaproteobacteria, bacteroidia, bacillus, actinobacteria were the dominant class. The bacteria with high abundances were Bacillus, rank-f-unclassifie, unclassified-f-Flavobacteriaceae, Aquibacter, unclassified-f-rhodobacteraceae, Nitrospira, Nitratireductor, Methylotenera, Methylophaga, Labrenzia. Nitrospira which mainly distributed in plant roots and fine sand layer, while denitrifying bacteria (such as Bacillus) mainly distributed in coal cinder layer and coral layer with low dissolved oxygen.