Characterization of volatile substances of different product formats of Chinese mitten crab (Eriocheir sinensis)
YIN Jing, GUAN Weibin, SHI Wei, WANG Jianglong, YU Xiaoyan, ZHANG Guangdi
Based on the visual characteristics demonstrated in the headspace-gas chromatography-ion mobility spectroscopy (HS-GC-IMS), the volatile substances of the muscle and gonad of Chinese mitten crab were determined for the fresh, the refrigerated and anesthetized, and the frozen products of the crab harvested from rice crab symbiotic system. The differences in the contents of these materials were revealed according to the fingerprints in the spectra through principal component analysis and with the matching matrices method. In total, 47 volatile substances were identified among three formats of products. The main volatile substances included ketones, aldehydes, alcohols, acids, esters and heterocyclic, of them the most important were 12 ketones, 9 aldehydes and 8 alcohols. In the muscles of fresh, refrigerated and anesthetized, and frozen products, 15, 19 and 22 volatile substances were identified. In the Chinese mitten crab gonad in different product formats, 17, 19 and 32 volatile substances were found, and some of them were shared among the product formats. The volatile substances (ketones, alcohols, aldehydes) increased with freezing. From the fresh to the anaesthetized to the frozen, a large number of aldehydes, furans, pyrazines among others produced in gonads and muscles, of these substances, 2-pentylfuran causes the quality decrease of Chinese mitten crab. The volatile substance variation and PCA of three formats of products can clearly distinguish the three formats. Our findings provides a reference for volatile substance evaluation, product format identification, deep processing, preservation and cold chain circulation of Chinese mitten crab.