Screening, identification and application of protease-producing strains in Antarctic krill meal

LI Xueping, LIU Zhidong, NING Xibin

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Transactions of Oceanology and Limnology ›› 2025, Vol. 47 ›› Issue (1) : 173-180. DOI: 10.13984/j.cnki.cn37-1141.2025.01.021
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Screening, identification and application of protease-producing strains in Antarctic krill meal

  • LI Xueping1, LIU Zhidong2, NING Xibin1,3,4
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Abstract

In order to improve the utilization rate of shrimp meal feed, in this study, we attempted to screen an acid-producing protease strain L-22 from the shrimp meal hydrolysate in combination with modern fermentation technology. Physiological, biochemical, and 16S rDNA identification of the strain was carried out. The sequences were compared with BLAST and use to construct a phylogenetic tree. The nucleic acid sequence was deposited into GenBank with an accession number through Bankit. The conditions that affected the fermentation of the strain were explored under liquid fermentation conditions. The results showed that the bacterium was a safe strain that can tolerate high temperature and high salt environments, and hydrolyze gelatin, starch, tyrosine among others. It was determined to be Bacillus subtilis. The molecular weight of its proteins as were shown in SDS-PAGE electrophoresis varied between 29.0 KDa and 44.3 KDa. Under liquid fermentation conditions, the free amino acid content and enzyme activity were used as indicators. The amino acid content in the fermentation broth changed from initial 4.29±0.259 mg/mL to 6.65±0.20 mg/mL, and the enzyme activity increased from 25.17±0.840 U/mL to 39.90±0.95 U/mL after a partial optimization of the conditions. The strain obtained has a good effect on improving the utilization rate of shrimp meal.

Key words

acid protease / separation / fermentation / Antarctic krill shrimp powder / amino acid

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LI Xueping, LIU Zhidong, NING Xibin. Screening, identification and application of protease-producing strains in Antarctic krill meal. Transactions of Oceanology and Limnology. 2025, 47(1): 173-180 https://doi.org/10.13984/j.cnki.cn37-1141.2025.01.021

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